siRNA / RNAi /miRNA transfection Rat A-10

Get tips on using Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) to perform ChIP Anti-bodies H3K27ac

Get tips on using Recombinant Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) to perform ChIP Anti-bodies ERα

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Anti-REG1 alpha antibody (ab231092) to perform Immunohistochemistry Mouse - Reg1

Get tips on using Anti-Choline Acetyltransferase Antibody AB5042 to perform Immunohistochemistry Mouse - ChAT

Get tips on using Anti-activated Notch1 antibody (ab8925) to perform Western blotting Notch1

Get tips on using Anti-LC3B antibody (ab48394) to perform Autophagy assay cell type - Cholangiocarcinoma

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform DNA Damage Assay HeLa

Get tips on using Anti-Apg7 antibody [EPR6251] to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Anti-EpCAM antibody [323/A3] (ab85987) to perform Immunohistochemistry Mouse - EpCAM