siRNA / miRNA gene silencing Human CAL-27

Get tips on using Growth Medium MV 2 to perform Mammalian cell culture media HAOECv

Get tips on using CnAOEC Growth Medium Kit to perform Mammalian cell culture media CnAOEC

Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media BAOSMC

Get tips on using MEM (Eagle) Liquid Medium to perform Mammalian cell culture media MDCK

Get tips on using Minimum Essential Media (MEM) to perform Mammalian cell culture media MDCK

Get tips on using Minimum Essential Media (MEM) to perform Mammalian cell culture media Vero

Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media Vero

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized CML-T1

Get tips on using MYH9 CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Rat - Deletion PC12 myosin IIA (Myh9)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.