Microarray Gene expression arrays A-375 human melanoma

Get tips on using Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody to perform Autophagy assay cell type - HepG2

Get tips on using FITC Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - SKOV3

Get tips on using Recombinant Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396) to perform Western blotting Estrogen Receptor

Get tips on using Anti-ATG5 (C-terminal) antibody produced in rabbit to perform Autophagy assay cell type - Rat spinal cord tissue

Get tips on using FITC Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - PANC-1

Get tips on using Dead Cell Apoptosis Kit with Annexin V FITC and PI to perform Apoptosis assay cell type - HUVEC

Get tips on using Dead Cell Apoptosis Kit with Annexin V FITC and PI to perform Apoptosis assay cell type - OECM-1

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Atg14 Antibody to perform Autophagy assay cell type - A2780

Get tips on using Atg5 Antibody to perform Autophagy assay cell type - A549