ChIP acH4 Human

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I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Discussions siRNA/RNAi/miRNA transfection human

Get tips on using RosetteSep™ Human T Cell Enrichment Cocktail to perform Cell Isolation Human T cells

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Get tips on using MagniSort™ Human T cell Enrichment Kit to perform Cell Isolation Human T cells

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Get tips on using EasySep™ Human T Cell Enrichment Kit to perform Cell Isolation Human T cells

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Get tips on using EasySep™ Human T Cell Isolation Kit to perform Cell Isolation Human T cells

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Get tips on using Human TGF beta 1 ELISA Kit (ab100647) to perform ELISA Human - TGF-beta 1

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Get tips on using Human TGF-beta 1 Quantikine ELISA Kit to perform ELISA Human - TGF-beta 1

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Get tips on using Human IL-1 beta ELISA Kit (ab100562) to perform ELISA Human - IL-1 beta

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Get tips on using PE anti-human CD114 (G-CSFR) Antibody to perform Flow cytometry Anti-bodies Human - CD114

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Get tips on using PE Mouse Anti-Human CD26 Clone L272 to perform Flow cytometry Anti-bodies Human - CD26

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