ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.
Get tips on using SurePrint Human miRNA Microarrays to perform Microarray Human - Endometrial stromal cells miRNA-expression array (labelled)
Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.
Get tips on using ON-TARGETplus Human CYP3A4 (1576) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Primary Human Hepatocytes CYP3A4
Get tips on using ON-TARGETplus Human CYP2B6 (1555) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Primary Human Hepatocytes CYP2B6
Get tips on using ON-TARGETplus human ATG16L1 siRNA to perform siRNA / miRNA gene silencing Human - SHSY5Y ATG16L1
Get tips on using ON-TARGETplus Human TET1 siRNA to perform siRNA / miRNA gene silencing Human - A172 TET1
Get tips on using ON-TARGETplus Human BECN1 siRNA to perform siRNA / miRNA gene silencing Human - U251 Beclin 1
Get tips on using Silencer® GAPDH siRNA (human) to perform siRNA / miRNA gene silencing Human - Jurkat GAPDH Lipid
Get tips on using Viromer® RED to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa
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