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Get tips on using Fast SYBR™ Green Master Mix to perform PCR Quantitative real-time PCR - Bacterial DNA

Get tips on using SYBR™ Green PCR Master Mix to perform PCR Quantitative real-time PCR - Mammalian DNA

What is the optimal concentration for primers in qPCR? My total volume is 20μl per reaction.

Get tips on using LightCycler® 480 SYBR Green I Master to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using Brilliant II SYBR Green qPCR Master Mix to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Renal carcinoma cell line Caki-1

Get tips on using LightCycler® FastStart DNA Master SYBR Green I to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Renal carcinoma cell line Caki-1

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.