DNA transfection Mammalian cells Immortalized cell lines

Get tips on using Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - K562

Get tips on using Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - HEK293

Get tips on using Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - L02

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Macrophages

Get tips on using Cytochrome c Releasing Apoptosis Assay Kit to perform Apoptosis assay cell type - SKOV3

Get tips on using EasySep™ Human CD33 Positive Selection Kit II to perform Cell Isolation Monocyte

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - L-02 human fetal hepatocyte

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - rat kidney and pancreas tissue

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.