rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

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Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates NHEK normal human epidermal keratinocytes

Get tips on using Rac1/2/3 Antibody #2465 to perform Western blotting Rac1

Products Cell Signaling Technology Rac1/2/3 Antibody #2465

Get tips on using Cell-APOPercentage™ Apoptosis Assay to perform Apoptosis assay cell type - RAW 264.7

Products Biocolor Cell-APOPercentage™ Apoptosis Assay

Get tips on using K-Ras Antibody (F234): sc-30 to perform Western blotting Ras

Products Santa Cruz Biotechnology K-Ras Antibody (F234): sc-30

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - RAW 264.7

Products Bio-Techne Cultrex® BME Cell Invasion Assay

Get tips on using Rab 7 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B RAB7A

Products Santa Cruz Biotechnology Rab 7 siRNA (h)

Get tips on using Rab 5C siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B RAB5C

Products Santa Cruz Biotechnology Rab 5C siRNA (h)

Get tips on using Anti-Rad51 antibody - ChIP Grade (ab176458) to perform ChIP Anti-bodies RAD51

Products Abcam Anti-Rad51 antibody - ChIP Grade (ab176458)

Get tips on using PDGF Receptor β (28E1) Rabbit mAb to perform Immunohistochemistry PDGFβR - Rabbit Mouse -NA-

Products Cell Signaling Technology PDGF Receptor β (28E1) Rabbit mAb

Get tips on using 53BP1 Antibody (H-300) rabbit polyclonal to perform Immunohistochemistry 53BP1 - Rabbit IgG Human -NA-

Products Santa Cruz Biotechnology 53BP1 Antibody (H-300) rabbit polyclonal

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