siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - HeLa cells

Get tips on using pET-INP-cellulase to perform Protein Expression Prokaryotic cells - E. coli INP-cellulase

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - HUVEC

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - HeLa

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - HepG2

Get tips on using Mammalian Cell Lysis kit to perform Protein isolation Mammalian cells - STTG1

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Proximal tubular cells (rPT)

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - prostate cancer PPC1 cells

Get tips on using Anti-ATG4B antibody produced in rabbit to perform Autophagy assay cell type - prostate cancer PPC1 cells