Protein Expression Prokaryotic cells B. bifidum

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Human - T47D

Get tips on using Brilliant II SYBR Green qPCR Master Mix to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using Senescence β-Galactosidase Staining Kit - Beyotime to perform Reporter gene assay β-galactosidase substrates - rat nucleus pulposus

Get tips on using Senescence β-Galactosidase Staining Kit - Beyotime to perform Reporter gene assay β-galactosidase substrates - SK-Hep-1

Get tips on using Ovation® RNA Amplification System V2 to perform Microarray RNA amplification & Labeling - Bovine olfactory nasal tissues Biotin

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using miRNeasy Serum/Plasma Advanced Kit (50) to perform RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat

Get tips on using Anti-Beta Catenin CTNNB1 Antibody Picoband™ (Monoclonal, 1F6) to perform Immunohistochemistry Human - β-catenin

Get tips on using Recombinant Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) to perform Immunohistochemistry Mouse - β-Catenin

Get tips on using Laminin β-2 Antibody (H-1): sc-133241 to perform Western blotting Laminin subunit Beta-2