Cell Culture Contamination Detection Kit

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - THP-1

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - HEK 293

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - Hepa-1

Get tips on using illustra GFX PCR DNA and Gel Band Purification Kits to perform

Get tips on using TransAM® ER Transcription Factor ELISA Kits to perform ELISA Human - Estrogen receptor (ESRs)

Get tips on using KAPA Stranded RNA-Seq Kits with RiboErase (HMR) to perform RNA sequencing Rat - Lung tissue

Get tips on using KAPA Stranded RNA-Seq Kits with RiboErase (HMR) to perform RNA sequencing Human - MCF-7

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Human blood Biotin

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using KAPA Stranded RNA-Seq Kits with RiboErase (HMR) to perform RNA sequencing Human - MDA-MB-231