Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using Beclin 1 Rabbit Polyclonal antibody to perform Autophagy assay cell type - A375

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HepG2

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using TriDye™ 1 kb Plus DNA Ladder to perform DNA Ladder 1 kb

Get tips on using Quick-Load® 1 kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using TrackIt™ 1 Kb Plus DNA Ladder to perform DNA Ladder 1 kb

Get tips on using ICAM-1 Antibody (G-5): sc-8439 to perform Western blotting ICAM-1

Get tips on using ICAM-1 Recombinant Rabbit Monoclonal Antibody (9H21L19) to perform Western blotting ICAM-1

Get tips on using Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) to perform Western blotting Caveolin-1

Get tips on using Recombinant Anti-Claudin 1 antibody [EPR9306] (ab180158) to perform Western blotting Cclaudin-1