shRNA gene silencing Mouse RGC-5

Get tips on using Lamin B1 Antibody (C-5): sc-365962 to perform Western blotting Lamin B

Get tips on using β-catenin Antibody (E-5): sc-7963 to perform Western blotting eta-catenin

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA quantification Human - BJ

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA quantification Human - WI-38

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA quantification Human - HEK 293

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA quantification Human - THP 1

Get tips on using QIAamp 96 DNA QIAcube HT Kit (5) to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAamp 96 PowerFecal QIAcube HT Kit (5) to perform DNA isolation / purification Micorbiome - Human gut

Get tips on using 5 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - A549