The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.
Get tips on using pJAP2 to perform Protein Expression Eukaryotic cells - HEK293 AT1R
Get tips on using pJAP34 to perform Protein Expression Eukaryotic cells - HEK293 A1R
Get tips on using pJAP37 to perform Protein Expression Eukaryotic cells - HEK293 A1R
Get tips on using PL_MmXbp1s to perform Protein Expression Eukaryotic cells - CHO Xbp1s
Get tips on using PL_MmPdia3 to perform Protein Expression Eukaryotic cells - CHO Pdia3
Get tips on using PL_MmHspa5 to perform Protein Expression Eukaryotic cells - CHO Hspa5
Get tips on using PL_MmHsp90b1 to perform Protein Expression Eukaryotic cells - CHO Hsp90b1
Get tips on using PL_MmEro1l to perform Protein Expression Eukaryotic cells - CHO Ero1l
Get tips on using PL_MmBax to perform Protein Expression Eukaryotic cells - CHO Bax
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment