rna-isolation-purification-cells-immortalized-ntera-2

Get tips on using Pierce™ LDH Cytotoxicity Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - HEK 293

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication MDA‐MB‐231 cell line

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - MDA-MB-231

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication MDA‐MB‐231 cell line

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Glioblastoma cell line LN-229

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line

Get tips on using Autophagy Assay Kit to perform Autophagy assay cell type - Beas-2B