A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.
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Get tips on using TurboCapture 96 mRNA Kit (5) to perform mRNA / Ribonucleoprotein isolation / purification mRNA
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Get tips on using Oligotex mRNA Midi Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA
Get tips on using Oligotex mRNA Mini Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA
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A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality hot-start DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction
Get tips on using PCR Sequencing Kit to perform Whole Genome Amplification Human
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