siRNA / miRNA gene silencing Human HeLa EPAS-1

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using YAP siRNA (h) to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene

Get tips on using ON-TARGETplus Human CFL1 (1072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 CFL1

Get tips on using ON-TARGETplus Human AHR (196) siRNA - Individual to perform siRNA / miRNA gene silencing Human - MDA-MB-231 AHR

Get tips on using ON-TARGETplus Human PAK1 (5058) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Caco-2 PAK1

Get tips on using ON-TARGETplus Human FOXM1 (2305) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MCF-7 FOXM1

Get tips on using ON-TARGETplus Human ERCC4 (2072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U2OS XPF/ERCC4

Get tips on using ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A431 RCP/RAB11FIP1

Get tips on using ON-TARGETplus Human SNAI2 (6591) siRNA - Individual to perform siRNA / miRNA gene silencing Human - HL-60 Slug

Get tips on using ON-TARGETplus Human BBC3 (27113) siRNA - Individual to perform siRNA / miRNA gene silencing Human - HL-60 puma