siRNA / miRNA gene silencing Human HNSCC cell line

Get tips on using CytoTune™-iPS 2.0 Sendai Reprogramming Kit to perform Stem cell Differentiation media Differentiation of RPE cells into hiPSC cells

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Gibco™IMDM to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Get tips on using Gibco™Neurobasal™ Medium to perform Stem cell culture media h-medial pallium induction and culture

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hiPSCs or hPSCs differentiation into trophoblasts

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE)

Get tips on using Gibco™Glasgow's MEM (GMEM) to perform Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE)

Get tips on using DMEM, high glucose, GlutaMAX™ Supplement, pyruvate to perform 3D Cell Culture Media hiPSC-derived retinal organoids

Get tips on using Gibco™DMEM/F-12, no glutamine to perform 3D Cell Culture Media hiPSC-derived lung organoids

Get tips on using Gibco™Ham's F-12 Nutrient Mix to perform Stem cell Differentiation media hESCs differentiation into SFEBq