siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Get tips on using RIPA Lysis Buffer System to perform Protein isolation Mammalian cells - HOG

Get tips on using RIPA Lysis Buffer, 10X to perform Protein isolation Mammalian cells - STTG1

Get tips on using 2-D Quant Kit to perform Protein quantification Mammalian cells - SiHa

Get tips on using DNeasy mericon Food Kit (50) to perform DNA isolation / purification Processed food

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Get tips on using GeneJET Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp

Get tips on using Brilliant Violet 510™ anti-mouse CD69 Antibody to perform Flow cytometry Anti-bodies Mouse - CD69

Get tips on using GenElute™ Bacterial Genomic DNA Kit to perform DNA isolation / purification Bacteria - Gram positive Actinomycytes

Get tips on using GenElute™ Bacterial Genomic DNA Kit to perform DNA isolation / purification Bacteria - Gram negative E.coli

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Rat_Mesenteric fat