Site Directed Mutagenesis (SDM) Human Deletion HepG2

Get tips on using ON-TARGETplus Human PPARGC1B siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 PGC-1β/PPARGC1B

Get tips on using ON-TARGETplus Human SMU1 (55234) siRNA - Individual to perform siRNA / miRNA gene silencing Human - HeLa SMU1

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using ON-TARGETplus Human ELMOD2 (255520) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa ELMOD2

Get tips on using ON-TARGETplus Human ARL3 (403) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa ARL3

Get tips on using ON-TARGETplus Human LRRC8A (56262) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A2780 LRRC8A

Get tips on using ON-TARGETplus Human CASP3 (836) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Huh7 CASP3

Get tips on using ON-TARGETplus Human RET (5979) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - TT RET

Get tips on using ON-TARGETplus Human EGFR (1956) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - TT EGFR

Get tips on using ON-TARGETplus Human KDR (3791) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - TT KDR