siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

Get tips on using ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - CAL-27 DPAGT1

Get tips on using ON-TARGETplus Human CTHRC1 (115908) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - CAL-27 CTHRC1

Get tips on using ON-TARGETplus Human SLC7A2 (6542) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Caco-2 SLC7A2

Get tips on using ON-TARGETplus Human PCSK7 (9159) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 PC-7

Get tips on using ON-TARGETplus Human PCSK7 (9159) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 IGFBP-7

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Accell Human VDAC1 (7416) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Human - HeLa VDAC1

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Get tips on using Accell Human VDAC1 (7416) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Human - U78MG VDAC1