Site Directed Mutagenesis (SDM) Human Point mutation THP-1

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

Get tips on using pTrcHis-TOPO to perform Protein Expression Prokaryotic cells - E. coli Murine BCO2

Get tips on using TopTaq Master Mix Kit to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using QIAexpress Type IV Kit to perform Protein tag Purification of His-tagged proteins

Get tips on using pUC19-Pgpd-xyn2-Tgpd to perform Protein Expression Eukaryotic cells - T. reesei xyn2

Does anyone know how “strong” the PCR product of methylation specific PCR is? I kept my PCR products at 4C for about 3 weeks and then at room temperature for another week. Will I be able to use them for sequencing?

Get tips on using Type-it HRM PCR Kit (2000) to perform High-resolution melting (HRM) analysis Bacterial

Get tips on using Anti-Collagen Type II Antibody, clone 6B3 to perform Immunohistochemistry Mouse - Col II

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.