Immunohistochemistry ATM phospho Rabbit IgG Human

Get tips on using anti-alpha-Smooth Muscle Actin mouse monoclonal, ASM-1 to perform Immunohistochemistry Alpha smooth muscle Actin - Mouse -NA- -NA-

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit aortic smooth muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rabbit aortic endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rabbit skeletal muscle cells

Get tips on using APO-BRDU™ Kit (RUO) to perform TUNEL assay cell type - Rabbit synovial fibroblasts

Get tips on using Purified Mouse Anti-SV40 Large T Antigen Clone PAb 101 (RUO) to perform Immunohistochemistry Mouse - SV40

Get tips on using Human IGF-1 PicoKine™ ELISA Kit to perform ELISA Human - IGF-I

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit skeletal muscle-derived stem cells

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.