siRNA / miRNA gene silencing Human MCF-7

Get tips on using siRNA P300 to perform siRNA / miRNA gene silencing Human - BOSC23 p300

Get tips on using siRNA CBP to perform siRNA / miRNA gene silencing Human - BOSC23 CBP

Get tips on using siRNA ADAM17 to perform siRNA / miRNA gene silencing Human - BOSC23 ADAM17

Get tips on using siRNA AURKA to perform siRNA / miRNA gene silencing Human - A549 AURKA

Get tips on using siRNA EGFR to perform siRNA / miRNA gene silencing Human - A431 EGFR

Get tips on using PACE4 siRNA (h) to perform siRNA / miRNA gene silencing Human - DuCaP

Get tips on using Silencer® FANCD2 siRNA (human) to perform siRNA / miRNA gene silencing Human - 501 Mel and SK Mel 28 FANCD2

Get tips on using MIF siRNA (h) to perform siRNA / miRNA gene silencing Human - Caki-2 MIF

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.