The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.
The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.
Get tips on using Cell Counting Kit-8 to perform RNA quantification Coloremetric
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Get tips on using FastDigest RsaI to perform Restriction Enzymes RsaI / AfaI
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Get tips on using RsaI (10 U/µL) to perform Restriction Enzymes RsaI / AfaI
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