Protein quantification Mammalian cells

Get tips on using IncuCyte® Caspase-3/7 Apoptosis Assay Reagent to perform Apoptosis assay cell type - Caspase 3/7

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using NA-Star™ Influenza Neuraminidase Inhibitor Resistance Detection Kit to perform Cell Culture Contamination Detection Kit Virus

Get tips on using Staphylococcal Enterotoxin Reversed Passive Latex Agglutination Kit (SET- RPLA) to perform Cell Culture Contamination Detection Kit Bacteria

Get tips on using BacTx® Rapid Test for Bacterial Contamination of Platelets to perform Cell Culture Contamination Detection Kit Bacteria

Get tips on using DMEM, no glucose, no glutamine, no phenol red to perform 3D Cell Culture Media hiPSC-derived cardiac organoids

Get tips on using Corning® Hepatocyte Culture Media Kit, 500 mL to perform 3D Cell Culture Media Human bladder cancer organoid

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform 3D Cell Culture Media hiPSC-derived retinal organoids

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform 3D Cell Culture Media hESC-derived brain organoids

Get tips on using Gibco™ DMEM, high glucose, GlutaMAX™ Supplement to perform 3D Cell Culture Media Mouse small intestinal organoids