Immunohistochemistry Anti-rabbit IgG Donkey

Get tips on using Anti-Smad2 (phospho S467) antibody (ab53100) to perform Western blotting Smad2

Get tips on using Anti-Oct4 antibody - ChIP Grade (ab19857) to perform Western blotting Oct4

Get tips on using Anti-Nanog antibody - ChIP Grade (ab21624) to perform Western blotting Nanog

Get tips on using Recombinant Anti-Paxillin antibody [Y113] (ab32084) to perform Western blotting Paxillin

Get tips on using Recombinant Anti-CDC42 antibody [EPR15620] (ab187643) to perform Western blotting Cdc42

Get tips on using Anti-AKT (phospho T308) antibody (ab38449) to perform Western blotting AKT

Get tips on using Recombinant Anti-p21 antibody [EPR362] (ab109520) to perform Western blotting p21

Get tips on using Recombinant Anti-JNK1 antibody [EPR17557] (ab199380) to perform Western blotting JNK

Get tips on using Anti-EGFR (phospho Y1068) antibody (ab5644) to perform Western blotting EGFR

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.