TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using Anti-alpha smooth muscle Actin antibody to perform Immunohistochemistry Alpha smooth muscle Actin - Rabbit Mouse -NA-
Get tips on using Rabbit monoclonal [EP1890Y] to ATM (phospho S1981) to perform Immunohistochemistry ATM phospho - Rabbit IgG Human -NA-
Get tips on using Rabbit Anti-Human CHK2 (NT) Affinity Purified pAb to perform Immunohistochemistry chk2 - Rabbit IgG Human -NA-
Get tips on using Purified Mouse Anti-E-Cadherin Clone 36/E-Cadherin (RUO) to perform Immunohistochemistry Human - E-Cadherin
Get tips on using SCGB1A1 antibody (Secretoglobin, Family 1A, Member 1 (Uteroglobin)) (Middle Region) to perform Immunohistochemistry Human - SCGB1A1 /CC10
Get tips on using Monoclonal Mouse Anti-Thyroid Transcription Factor (Concentrate) Clone 8G7G3/1 to perform Immunohistochemistry Human - TTF-1
Get tips on using Purified Mouse Anti-β-Catenin Clone 14/Beta-Catenin (RUO) to perform Immunohistochemistry Human - β-catenin
Get tips on using Monoclonal Mouse Anti-Human p53 Protein (Dako Omnis) Clone DO-7 to perform Immunohistochemistry Human - p53
Get tips on using Purified Mouse Anti-SV40 Large T Antigen Clone PAb 101 (RUO) to perform Immunohistochemistry Mouse - SV40
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment