The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Raji
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Calu-3
Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads could be the best alternative.
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized NHA
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Daudi
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized 59M
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized LoVo
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