rna-isolation-purification-cells-primary-mouse-cortical-neurons

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Apoptosis and Necrosis Quantification Kit to perform Necrosis MIA PaCa-2

Get tips on using GFP-CERTIFIED® Apoptosis/Necrosis detection kit to perform Necrosis HeLa

Get tips on using GFP-CERTIFIED® Apoptosis/Necrosis detection kit to perform Necrosis MCF7

Get tips on using GFP-CERTIFIED® Apoptosis/Necrosis detection kit to perform Necrosis A549

Get tips on using GFP-CERTIFIED® Apoptosis/Necrosis detection kit to perform Necrosis HCT 116

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis LNCaP

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis HUVEC