Immunohistochemistry CD31 Rat Mouse

Get tips on using APC anti-mouse CD117 (c-kit) Antibody to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit

Get tips on using PE anti-mouse CD138 (Syndecan-1) Antibody to perform Flow cytometry Anti-bodies Mouse - CD138/Syndecan-1

Get tips on using Purified anti-mouse CD138 (Syndecan-1) Antibody to perform Flow cytometry Anti-bodies Mouse - CD138/Syndecan-1

Get tips on using FITC Mouse Anti-Human CD36 to perform Flow cytometry Anti-bodies Human - CD36/CB38

Get tips on using Purified Mouse Anti-Human CD36 to perform Flow cytometry Anti-bodies Human - CD36/CB38

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.