Reporter gene assay

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MIA PaCa-2

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MDA-MB-231

Get tips on using Cell-APOPercentage™ Apoptosis Assay to perform Apoptosis assay cell type - RAW 264.7

Get tips on using Microbial Viability Assay Kit-WST to perform Live / Dead assay bacteria - Listeria innocua

Get tips on using Microbial Viability Assay Kit-WST to perform Live / Dead assay bacteria - Staphylococcus epidermidis

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hiPSC-2-EC

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hiPSC-1-EC

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay mouse - spleen-derived EPCs

Get tips on using Guava Nexin Annexin V Assay to perform Apoptosis assay cell type - HeLa cells

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.