siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells

Get tips on using siRNA MXD3 to perform siRNA / miRNA gene silencing Human - DAOY MXD3

Get tips on using siRNA FOXS1 to perform siRNA / miRNA gene silencing Human - DAOY FOXS1

Get tips on using siRNA P300 to perform siRNA / miRNA gene silencing Human - BOSC23 p300

Get tips on using siRNA CBP to perform siRNA / miRNA gene silencing Human - BOSC23 CBP

Get tips on using siRNA ADAM17 to perform siRNA / miRNA gene silencing Human - BOSC23 ADAM17

Get tips on using siRNA AURKA to perform siRNA / miRNA gene silencing Human - A549 AURKA

Get tips on using siRNA EGFR to perform siRNA / miRNA gene silencing Human - A431 EGFR

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using PACE4 siRNA (h) to perform siRNA / miRNA gene silencing Human - DuCaP

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.