Protein Expression Prokaryotic cells M. smegmatis

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Bacteria - Gram positive Listeria monocytogens

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Hippocampus

Get tips on using TopTaq Master Mix Kit to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using MMP-3 (D7F5B) Rabbit mAb #14351 to perform Immunohistochemistry Rat - MMP3

Get tips on using Human Myeloperoxidase ELISA Kit (MPO) (ab119605) to perform ELISA Human - MPO

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using MMP13 Recombinant Rabbit Monoclonal Antibody (3H13L17) to perform Western blotting MMP-13

Get tips on using MMP2 Monoclonal Antibody (F14 P4 D3) to perform Western blotting MMP-2

Get tips on using NucleoBond® Xtra Midi / Maxi to perform Plasmid Isolation Medicago truncatula BAC clone