Protein Expression Prokaryotic cells C. crescentus JS1014

Get tips on using Human IL-3R alpha /CD123 PE-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Get tips on using PE-Cy™7 Mouse Anti-Human CD123 to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Get tips on using CD206 (MMR) Monoclonal Antibody (19.2), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD206

Get tips on using PE-Cy™7 Rat Anti-Mouse CD31 to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1

Get tips on using CD45 Monoclonal Antibody (30-F11), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD45

Get tips on using CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD274/PD-L1

Get tips on using CAG-Cas9 to perform CRISPR Mouse - Deletion Neuro 2a Rab38

Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Rat - Heart

Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Mouse - Osteoblasts

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.