Protein Expression Eukaryotic cells P. pastoris

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using QIAamp PowerFecal Pro DNA Kit (50) to perform DNA isolation / purification Tissue - fecal sample

Get tips on using PureLink™ HiPure Plasmid Filter Midiprep Kit to perform Plasmid Isolation Enterobacter aerogenes

Get tips on using PureYield™ Plasmid Miniprep System Technical Bulletin to perform Plasmid Isolation Agrobacterium tumefaciens

Get tips on using RPM Yeast Plasmid Isolation Kit, 100 preps to perform Plasmid Isolation S. cerevisiae

Get tips on using Total RNA Purification Plus Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro cholerae

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pneumoniae

Get tips on using Purified Mouse Anti-Nucleoporin p62 to perform Western blot p62/SQSTM1 - Mouse IgG2b Human -NA-

Get tips on using DNeasy PowerSoil HTP 96 Kit (384) to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas