siRNA / RNAi /miRNA transfection Human Cells HNSCC

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Purification of extracellular vesicles Exosomes - Plasma Experiment

Cellular assays Purification of extracellular vesicles Exosomes Plasma

Anti Type X Collagen (raised against rat) pAb (Rabbit, Antiserum) Product

Get tips on using Anti Type X Collagen (raised against rat) pAb (Rabbit, Antiserum) to perform Immunohistochemistry Mouse - Col X

Products Cosmo Bio Anti Type X Collagen (raised against rat) pAb (Rabbit, Antiserum)

Purification of extracellular vesicles Exosomes - Seminal plasma Experiment

Cellular assays Purification of extracellular vesicles Exosomes Seminal plasma

Amino Allyl MessageAmp™ II aRNA Amplification Kit Product

Get tips on using Amino Allyl MessageAmp™ II aRNA Amplification Kit to perform RNA amplification & labeling Fish - Total RNA, Fundulus heteroclitus Cyanine 3 & 5

Products Thermo Fisher Scientific Amino Allyl MessageAmp™ II aRNA Amplification Kit

cDNA conversion with low concentrations Discussion

I have extracted RNA from brain tissue but my RNA concentrations are as low as 5ng/ml with my highest being around 80ng/ml. Will I be able to perform cDNA conversion with concentrations as low as these?

Discussions cDNA conversion with low concentrations

Will presence of EDTA effect cDNA synthesis Discussion

I intend to use iScript cDNA Synthesis Kit in order to synthesize cDNA for qPCR. I have confirmed that my RNA is pure however, according to my extraction protocol I have suspended the RNA in TE buffer containing 1mM EDTA. Will the presence of EDTA have an effect on cDNA synthesis?

Discussions Will presence of EDTA effect cDNA synthesis

cDNA synthesis Bacteria - Experiment

RNA cDNA synthesis Bacteria

Whole Transcriptome Amplification Virus Experiment

RNA Whole Transcriptome Amplification Virus

Microarray Mice - Cochlea Expression array (labelled) Experiment

RNA Microarray Mice Cochlea Expression array (labelled)

QuantiTect Virus Kit Product

Get tips on using QuantiTect Virus Kit to perform RNA quantification qPCR

Products Qiagen QuantiTect Virus Kit

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