Protein Expression Eukaryotic cells P. pastoris

Get tips on using PCR SuperMix to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using Agencourt AMPure XP - PCR Purification to perform

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

Get tips on using GeneJET Plasmid Miniprep Kit to perform Plasmid Isolation Piscirickettsia salmonis

Get tips on using CompactPrep Plasmid Midi Kit to perform Plasmid Isolation Yersinia pestis

Get tips on using RNAprep pure Kit to perform RNA isolation / purification Tissue - Human Placenta

Get tips on using DreamTaq DNA Polymerases to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using Biotools DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Activation RAW 264.7 Dmd