Protein Expression Prokaryotic cells A. cellulolyticus

Get tips on using pUC19/Pr-EC to perform Protein Expression Eukaryotic cells - K. lactis α-l-Fucosidase

Get tips on using pPIC9-His-FIP-Ganoderma to perform Protein Expression Eukaryotic cells - P. pastoris Ganoderma FIP

Get tips on using pVL1392-RIP1 8-322-His to perform Protein Expression Eukaryotic cells - S. frugiperda RIP1

Get tips on using pIVEX-GAA-omega-PHACTR1-H to perform Protein Expression Eukaryotic cells - N. benthamiana PHACTR1

Get tips on using pHR-CMV-TetO2-VSV-G to perform Protein Expression Eukaryotic cells - HEK293 VSV-G

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using Freedom™ DG44 Kit to perform Protein expression and purification Mammalian cells - CHO-K1 G12

Get tips on using pGAPZA-α-MF-op-rLlAlp2 to perform Protein Expression Eukaryotic cells - P. pastoris Alkaline phytase

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.