shrna-gene-silencing-human-islets-of-langerhans-zeb2-lentiviral-particles

Get tips on using ON-TARGETplus Human GAPDH (2597) siRNA - Individual to perform siRNA / miRNA gene silencing Human - Calu-3 GAPDH

Get tips on using ON-TARGETplus Human ITGB4 (3691) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - CAL-27 ITGB5

Get tips on using ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - CAL-27 DPAGT1

Get tips on using ON-TARGETplus Human CTHRC1 (115908) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - CAL-27 CTHRC1

Get tips on using ON-TARGETplus Human SLC7A2 (6542) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Caco-2 SLC7A2

Get tips on using ON-TARGETplus Human PCSK7 (9159) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 PC-7

Get tips on using ON-TARGETplus Human PCSK7 (9159) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 IGFBP-7

Get tips on using ON-TARGETplus Human THBS2 siRNA to perform siRNA / miRNA gene silencing Human - Aortic smooth muscle cell TSP-2

Get tips on using SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit to perform Microarray Human - PCOS

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.