siRNA / miRNA gene silencing Human Jurkat GAPDH

Get tips on using Hs_PLK1_7 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - A172 PLK1

Get tips on using Hs_HES6_3 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - A172 HES6

Get tips on using Hs_HES6_1 FlexiTube siRNA. to perform siRNA / miRNA gene silencing Human - A172 HES6

Get tips on using Hs_HDAC5_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - U937 HDAC5

Get tips on using CD151 siRNA (h) to perform siRNA / miRNA gene silencing Human - U251 CD151

Get tips on using FAK siRNA (h) to perform siRNA / miRNA gene silencing Human - U251 FAK

Get tips on using ON-TARGETplus Human EGR1 (1958) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Human - HCT15 Egr-1

Get tips on using SignalSilence® PTEN siRNA to perform siRNA / miRNA gene silencing Human - A2780 PTEN

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using ON-TARGETplus Human ITGB3 (3690) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 β3 integrin/ITGB3