siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4

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Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells

Products Epigentek MethylFlash Methylated DNA 5-mC Quantification Kit

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

Discussions Live/dead assay Bacteria

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

DNA DNA isolation / purification Cells Primary cells Rat astrocytes

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

DNA DNA isolation / purification Cells Primary cells Mouse embryonic fibroblast (MEF)

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

DNA DNA isolation / purification Cells Primary cells Rat cortical neurons

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Retinal ganglion cells (RGCs)

Products Illumina TruSeq Stranded mRNA
pET-32c Product

Get tips on using pET-32c to perform Protein Expression Prokaryotic cells - E. coli rpf-like protein

Products R Verma, Bacteriology and Mycology Division, Mycobacteria Labora pET-32c
Maj-NPLP Product

Get tips on using Maj-NPLP to perform Protein Expression Prokaryotic cells - E. coli M. japonicus neuroparsin-like peptide

Products Naoaki Tsutsui, Faculty of Science, Ushimado Marine Institute, O Maj-NPLP

Get tips on using Cytotoxicity Detection Kit (LDH) to perform Live / Dead assay mammalian cells - RAW 264.7

Products Sigma-Aldrich Cytotoxicity Detection Kit (LDH)

Get tips on using rMaj-pCHH-B to perform Protein Expression Prokaryotic cells - E. coli M. japonicus CHH-like peptide

Products Naoaki Tsutsui, Faculty of Science, Ushimado Marine Institute, O rMaj-pCHH-B

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