Protein Expression Eukaryotic cells P. pastoris

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

Get tips on using QuantiNova SYBR Green PCR Kit (2500) to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using QuantiNova IC Probe Assay Red 650 (500) to perform PCR Multiplex PCR - Viral

Get tips on using QuantiTect Multiplex RT-PCR NR Kit (1000) to perform PCR Multiplex PCR - Viral

Get tips on using CD41-PE, P2, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD41

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus saprophycitius

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis