DNA isolation / purification Yeast - Cryptococcus neoformans

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

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4 years ago

4 years ago by Jean Rossignol France

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

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Upstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.
Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
Downstream tips
- Include RNAse treatment for 15-20 min.
- Use prewarmed TE buffer to elute the DNA
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