Protein Expression Prokaryotic cells M. smegmatis

Get tips on using EpiTect MSP Kit to perform DNA methylation profiling Gene specific profiling - Ca Ski MEG3

Get tips on using Infinium MethylationEPIC Kit to perform DNA methylation profiling Gene specific profiling - MCF-7 LINE1

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Mucin 2 Antibody (Ccp58): sc-7314 to perform Immunohistochemistry Mouse - Muc-2

Get tips on using Cyclin E1 (HE12) Mouse mAb #4129 to perform Western blotting Cyclin E

Get tips on using MEK-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - WI-38 MEK1

Get tips on using EZ DNA Methylation-Direct Kit to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using EZ DNA Methylation-Gold Kit to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using EpiTect MSP Kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue MEG3

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse iPSCs