ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α

Get tips on using Human ANGPTL3 (highly sensitive) Assay Kit (27750 ) to perform ELISA Human - Angiopoietin-Like 3 (AngptL3)

Get tips on using pHYαCGT7 to perform Protein Expression Prokaryotic cells - B. subtilis α-CGTase

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using APC anti-mouse CD140a Antibody to perform Flow cytometry Anti-bodies Mouse - CD140/PDGFR-α

Get tips on using PE Rat Anti-Mouse CD140A to perform Flow cytometry Anti-bodies Mouse - CD140/PDGFR-α

Get tips on using pET-28a(+)-g-α13 to perform Protein Expression Prokaryotic cells - E. coli α-13 giardin

Get tips on using pUC19/Pr-EC to perform Protein Expression Eukaryotic cells - K. lactis α-l-Fucosidase

Get tips on using SEC-GFP-pHis1522 to perform Protein Expression Prokaryotic cells - Brevibacillus choshinensis SP3 α-amylase

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.