ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

Get tips on using Multi-Analyte ELISArray Kits to perform ELISA Human - GM-CSF

Get tips on using CD140a (PDGFRA) Monoclonal Antibody (APA5), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD140/PDGFR-α

Get tips on using ON-TARGETplus Rat Arhgef6 (363509) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 α-Pix/Arhgef6

Get tips on using ON-TARGETplus Mouse Nr1h3 (22259) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - M210B4 LXR‐α/Nr1h3

Get tips on using siRNA ILK to perform siRNA / miRNA gene silencing Human - CAL-27 ILK

Get tips on using MUC6 Polyclonal Antibody to perform Immunohistochemistry Human - MUC-6

Get tips on using Muc Glycoprotein Antibodies to perform Immunohistochemistry Human - MUC-6

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using LC3B antibody to perform Autophagy assay cell type - IEC-6