siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

Get tips on using PolyATtract® mRNA Isolation Systems to perform RNA isolation / purification Yeast - Coprinus cinereus

Get tips on using Zombie Violet™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - mouse microglia

Get tips on using Zombie UV™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - mouse splenocytes

Get tips on using Zombie Fixable Viability™ Sampler Kit to perform Live / Dead assay mammalian cells - HEK 293

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Live / Dead assay mammalian cells - CHO-K1

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Live / Dead assay mammalian cells - mouse bone marrow-derived macrophages

Hello fellow Labettors, I would like to know what would be the best method/medium to reactivate my dormant Lactobacillus paracasei cells?. Any suggestions are greatly appreciated.

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.