Found 2 discussions for this experiment
1 year ago
1 year ago by Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
2 years ago
2 years ago by Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Found 4 matching solutions for this experiment
|- This kit purifies RNA from a maximum amount of 30 mg fresh or frozen tissue or 20 mg of tissue that has been stabilized with RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent can generally be processed.|
|- Perform all centrifugation steps at 20–25ºC in astandard microcentrifuge|
|- For low A260/A280 value use 10 mM Tris·Cl,* pH 7.5, not RNase-free water, to dilute the sample before measuring purity.|