Protein Expression Eukaryotic cells T. thermophila

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - eye

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - lung

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - brain

Get tips on using SuperScript® II Reverse Transcriptase to perform cDNA synthesis Tissue

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using TaaI (HpyCH4III) (10 U/µL) to perform Restriction Enzymes TaaI / HpyCH4III

Get tips on using Tru1I (MseI) (10 U/µL) to perform Restriction Enzymes MseI / Tru1I

Get tips on using TLR4 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Neuro 2a TLR4

Get tips on using TGF-beta Pan Specific Antibody to perform Western blotting TGF-beta1